- 聯(lián)系人 : 封女士
- 聯(lián)系電話 : 15057010416
- 移動電話 : 15057010416
- QQ : 2880725808
|
貨號 |
產(chǎn)品名稱 |
規(guī)格（個/盒） |
|
80286 |
μ-Slide 2孔腔室載玻片，ibiTreat底部處理 |
15 |
|
80282 |
μ-Slide 2孔腔室載玻片，Collagen IV底部處理 |
15 |
|
80283 |
μ-Slide 2孔腔室載玻片，Fibronectin底部處理 |
15 |
|
80284 |
μ-Slide 2孔腔室載玻片，Poly-L-Lysine底部處理 |
15 |
|
80285 |
μ-Slide 2孔腔室載玻片，Poly-D-Lysine底部處 |
15 |
|
80281 |
μ-Slide 2孔腔室載玻片，無包被 |
15 |
|
80287 |
μ-Slide 2孔腔室載玻片，玻璃底 |
15 |
μ兩孔培養(yǎng)載玻片
用于**顯微鏡和**熒光的開放式μ兩孔培養(yǎng)載玻片
一體化小室，既可用于細(xì)胞培養(yǎng)，也可用于顯微鏡成像
使用高分辨率顯微鏡可以通過玻璃片底部進行樣品觀察
沒有蓋玻片，不會泄露，用于快速，簡單的**熒光。
只需少量細(xì)胞和試劑，降低了實驗成本
應(yīng)用:
細(xì)胞培養(yǎng)以及細(xì)胞培養(yǎng)物的顯微鏡觀察
轉(zhuǎn)染分析
活細(xì)胞和固定細(xì)胞的**熒光顯微鏡觀察
活細(xì)胞長時間段的成像
技術(shù)特征:
開放式載玻片，具有兩個獨立的培養(yǎng)孔
具備適用于**顯微鏡觀察的優(yōu)良光學(xué)成像特質(zhì)
兼容染色，固定
采用*佳細(xì)胞粘附的表面處理Ibitreat
生物兼容性塑料生產(chǎn)，無膠水，不泄露
規(guī)格:
|
孔數(shù)量 |
2 |
每孔生成面積 |
4.8 cm2 |
|
孔尺寸大小 |
21.2 x 23.3 x 9.3 |
每孔包被面積 |
7.5 cm2 |
|
每孔推薦體積 |
1500 μl |
底部配套的蓋玻片 |
No. 1.5 |
|
帶蓋子高度 |
10.8 mm |
底部 |
ibidi 標(biāo)準(zhǔn)底部 |
相差顯微鏡是*常用的，細(xì)胞培養(yǎng)中的透射光學(xué)技術(shù)。使用相差顯微鏡時，把兩個相位環(huán)彼此調(diào)節(jié)好是非常重要的。在開放式培養(yǎng)孔中，空氣-水交界面處的彎月牙就像一個棱鏡折射出光路。這個會誤校相位環(huán)，造成顯微鏡圖像的對比性較差。
使用μ- 2孔ph+培養(yǎng)載玻片減少了彎月牙，無論孔在哪個位置，整個光學(xué)系統(tǒng)都是平行的
μ-Slide 2 well
μ-Slide 2 well Ph+
The μ-Slide 2 well Ph+ (Phase Contrast +) is designed for excellent optical quality, especially for cell culture when normal phase contrast microscopy is being used. Opposed to the classic μ-Slide 2 well, the Ph+ version provides a special intermediate plate in the center of the well. This plate flattens the meniscus that disturbs the phase contrast effect in normal open wells. Openings near the corners provide access to the wells for easy filling and aspiration of liquids.
This innovative technique supports meniscus-free phase contrast microscopy in a very convenient manner.
Cross Section Through a Well of the μ-Slide 2 well Ph+ with a Transmitted Light Beam Path
The illustration on the left shows the perturbing effect of a meniscus. Light is refracted on the air-water-interface, leading to poor contrast in microscopy. Only the small center part exhibits satisfying phase contrast.
Working with the μ-Slide 2 well Ph+ diminishes the meniscus and increases the area of nicely contrasted cells. This nice contrast is due to the parallel beam path that is created by the plate.
